PhosphoFlow Analysis

 Phosphoflow cytometric assays can specifically detect the state of protein phosphorylation at the cellular level. Our assays are optimized for multiple cell types including immune cells and whole bloods.

A general workflow is as follows:

1. Treated and control cells or tissue/blood samples are processed and stained with viability dye and cell surface marker antibodies.
2. The cells are fixed and permeabilized.
3. The cells are then stained with the antibodies for intracellular phosphoproteins.
4. The cells are acquired on a flow cytometer.

•  Minuscule samples (minimal volume)
• Compatibility with heterogeneous samples: works with a variety of sample types such as cultured cells, whole blood, PBMCs, isolated tissue cells (such as skin cells), etc.

• Superior specificity: delivers greater specificity than other common technologies like Western blot, potentially revealing heterogeneity in samples.
• Flexibility: easily automated for use in routine compound screening
• Simultaneous detection: detect multiple targets (phosphoproteins) and other markers in the same cell type or in distinct cell subsets in a heterogeneous cell sample.
• Standardized platform: 96-well plate format using Cytoflex S flow cytometer.
• Use of specialized reagents: Assay conditions are optimized for accurate and reproducible data.
• Measure phosphorylation at a single cell
• Provides data for mechanistic insights.

• High throughput: 96-well format
• Simultaneous quantitation: detect phosphoproteins in multiple cell populations in the same sample and/or multiple phosphoproteins in the same cell type
• Highly reproducible
• State-of-art platforms: Cytoflex S flow cytometer
• Large inventory of phosphoprotein specific antibodies available
• Extensive data analysis
• Timely data delivery: 1-4 weeks or sooner, dependent upon receiving test samples
• 20+ years of experience: Expert data analysis and interpretation, high quality scientific and technical support