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    • Our Company
    • Immunoassay
    • Immune Cell Assay
    • Flow Cytometry
    • Discovery Biology
    • Products
    • Our Advantages
    • Contact Us
  • Our Company
  • Immunoassay
  • Immune Cell Assay
  • Flow Cytometry
  • Discovery Biology
  • Products
  • Our Advantages
  • Contact Us

DISCOVERY BIOLOGY & PHARMACOLOGY SERVICES

We are committed to your success of drug discovery

In Vitro Drug Screening

Translational Development

Translational Development

high-through put compound screening; 
cancer drug screening

  • Cytotoxicity and proliferation assays: MTT/XTT/SRB/Cell-titer Glo/ Almar Blue/BrDU/Calcein-AM; 3 D cell culture; cell cycle analysis; microtubule and mitosis test
  • Angiogenesis assays: endothelial cell (EC) proliferation, adhesion, migration, invasion, chemotaxis, tubule formation, aorta ring explant, VEGFR signaling
  • Epigenetics assays: Acetyltransferase, DNA Methyltransferase, HDAC, Histone Demethylase, Histone Methyltransferase, Bromodomain, PARP screening and profiling 
  • Cell free assays: various kinase, phosphatase, and other enzymatic assays 
  • Reporter gene assays 
  • Analysis of post-translational modification of protein
  • Screening of anti-aging agents for cosmetics

Translational Development

Translational Development

Translational Development

  • Biomarker validation and measurement using SimpleWestern
  • Characterization of mechanism of action of novel drugs
  • Target identification and validation of drug candidates and novel compounds
  • Drug combination screening for synergistic studies
  • Development of companion diagnostic methods
  • Simultaneous multiplex analysis gene and protein expression. 


PROTAC Discovery

Translational Development

Live Cell Imaging

PROTAC discovery; 
PROTAC; 
PROATAC optimization; 
ligase 3 assay

Based upon our decades of expertise of discovery of immunomodulatory drugs (IMiDs), we help:

  • Identify and characterize new, potent, and selective ligands that bind and reprogram E3 ligase substrate specificity with our ligand binding assay.
  • Develop, characterize, and validate the warhead end of novel PROTACs.
  • Enable sensitive quantitation of PROTAC-mediated degradation of targets for DC50 and Dmax values 
  • Evaluate the impact of target degraders on efficacy and safety-related translational biomarkers with our automatic western blot, IHC and other platforms.
  • Characterize PROTAC selectivity across the proteome and provide a global readout on changes in protein expression using protein microarray such as RPPA.
  • Provide on-shelf assays, such as CRBN, BET-VHL binding assay,   VHL and CRBN ternary complex formation assay

Live Cell Imaging

Gene and Protein Microarray

Live Cell Imaging

live cell imaging; 
high content screening

  •  Cell adhesion, migration, chemotaxis and wound healing 


Gene and Protein Microarray

Gene and Protein Microarray

Gene and Protein Microarray

QuantiGene assay; 
RPPA; 
Multiplex ELISA; 
Protein microarray

  •  Genome-wide gene array, low density gene array, real-time RT-PCR 

3-D In Vitro Models

Gene and Protein Microarray

Gene and Protein Microarray

3-D cell culture model

  • 3-D anti-psoriatic drug screening 
  • 3-D cancer cell viability assay  
  • 3-D spheroid cell invasion assay
  • 3-D tumor micro-tissue assay
  • 3-D anti-skin aging cosmetic ingredient screening

Animal Model for Cancers

Animal Model for Inflammation

Animal Model for Inflammation

xenograft animal model

  •  Mouse tumor models using patient cancer cells or cell lines (PDX or xenograft models) 
  • Angiogenesis models using mice and zebra-fish
  • Microbiopsy sampling and real-time monitoring efficacy and toxicity of drugs

Animal Model for Inflammation

Animal Model for Inflammation

Animal Model for Inflammation

inflammation model

  • Topical arachidonic acid-induced inflammation model (rodent)
  • Adjuvant-induced rheumatoid arthritis model (rodent  AIA)
  • Collagen-induced arthritis models (CIA)
  • Experimental Autoimmune Encephalomyelitis model (rodent EAE)
  • OVA-induced asthma rodent model
  • Pristane-Induced Systemic Lupus Erythematosus model (mouse SLE)
  • MRL/lpr genetic Systemic Lupus Erythematosus model (mouse SLE)
  • Inflammatory Bowel Disease models (rodent IBD)
  • Delayed-Type Hypersensitivity model (rodent DTH)
  • FITC Induced Dermatitis mouse model
  • Imiquimod-induced Psoriasis model
  • Bleomycin-induced Pulmonary Fibrosis mouse model

In vitro Toxicology/Safety Test

Animal Model for Inflammation

In vitro Toxicology/Safety Test

  • Analysis of DNA damage (comet, DNA fiber assay), DNA oxidation; DNA methylation 
  • Ames test (bacterial reverse mutation, 2 or 5 strains
  • Micronucleus test (MNT)
  • Mitochondrial toxicity
  • CFU assay for hematotoxicity assessment
  • Colony-forming cell-fibroblast (CFU-F) assay for mesenchymal toxicity assessment
  • Cell-specific cytotoxicity

A wide range of assays and cancer cell collection for compound profiling:

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